prfA -Like Transcription Factor Gene lmo0753 Contributes to l -Rhamnose Utilization in Listeria monocytogenes Strains Associated with Human Food-Borne Infections

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PrfA-like transcription factor gene lmo0753 contributes to L-rhamnose utilization in Listeria monocytogenes strains associated with human food-borne infections.

Listeria monocytogenes is a food-borne bacterial pathogen and the causative agent of human and animal listeriosis. Among the three major genetic lineages of L. monocytogenes (i.e., LI, LII, and LIII), LI and LII are predominantly associated with food-borne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp/Fnr family transcript...

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A prfA-like transcription factor gene lmo0753 contributes to L-rhamnose

19 Listeria monocytogenes is a foodborne bacterial pathogen and the causative agent of 20 human and animal listeriosis. Among the three major genetic lineages of L. monocytogenes (i.e. 21 LI, LII, and LIII), LI and LII are predominantly associated with foodborne listeriosis outbreaks, 22 whereas LIII is rarely implicated in human infections. In a previous study, we identified a 23 Crp/Fnr famil...

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Sigma B contributes to PrfA-mediated virulence in Listeria monocytogenes.

Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp(1) and prfAp(2)) or from the upstream plcA promoter. We demonstrate that prfAp(2) is a functional sigma(B)-dependent promoter and that a sigB deletion mutation affects the virulence phenotype of L. monocytogenes. Thus, the alternat...

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Rhamnose-Inducible Gene Expression in Listeria monocytogenes

Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in ...

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Restriction enzyme analysis of Listeria monocytogenes strains associated with food-borne epidemics.

Listeria monocytogenes (serotype 4b) has caused four major food-borne epidemics in North America. In this study, L. monocytogenes isolates from the Nova Scotia (Canada), Boston (Mass.), and Los Angeles (Calif.) outbreaks were examined by restriction enzyme analysis with the endonuclease HhaI. Human isolates (n = 32) from the 1981 Canadian outbreak were compared with a strain recovered from cole...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2013

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.01812-13