Prevalence Study of Biofilm Marker ica D Gene among Coagulase Positive Staphylococcus of Mastitic Origin

Bovine Mastitis is an inflammation of the mammary gland, usually due to microbial infection, which causes economic loss dairy producer every year. Staphylococcus one most common pathogens responsible for contagious mastitis in bovines. The aim this study was identify coagulase positive Staphylococci isolated from bovine subclinical mastitis. In vitro biofilm forming ability isolates, important virulence factor mastitogen and prevalence marker icaD gene among isolates. present study, 70 isolates were biochemically identified as S. aureus. Biochemically characterized strains aureus screened formation. Phenotypic detection formation S.aureus done by two methods, namely, Congo Red Agar (CRA) method Tube Method (TM). frequency 28.57 per cent on CRA plates while 71.42 non-producers. 38.57 total shown tube 61.42 Employing PCR, molecular technique carried out. 72.85 found be produced amplicon 381bp.