Prevalence of acquired AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes at a Spanish hospital from 1999 to 2007

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Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999-2007.

OBJECTIVES To gain insights into ampC transmission between bacterial strains. METHODS We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization. I-CeuI/PFGE was used for isolates not characterized by plasmid analysis. PCR...

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Prevalence of quinolone resistance mechanisms in Enterobacteriaceae producing acquired AmpC β-lactamases and/or carbapenemases in Spain.

BACKGROUND Quinolone resistance in Enterobacteriaceae species has increased over the past few years, and is significantly associated to beta-lactam resistance. The aim of this study was to evaluate the prevalence of chromosomal- and plasmid-mediated quinolone resistance in acquired AmpC β-lactamase and/or carbapenemase-producing Enterobacteriaceae isolates. METHODS The presence of chromosomal...

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AmpC-BETA Lactamases among Enterobacteriaceae Isolated at a Tertiary Hospital, South Western Uganda

AIM To characterize AmpC-beta lactamases among Enterobacteriaceae isolates from clinical samples at Mbarara Regional Referral Hospital. STUDY DESIGN Laboratory-based descriptive cross-sectional study. PLACE AND DURATION OF STUDY Microbiology Department, Mbarara Regional Referral Hospital and MBN clinical Laboratories, between May to September 2013. METHODOLOGY This study included 293 Ente...

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AmpC β lactamases in Urinary Klebsiella pneumoniae Isolates: First Report of ACC Type AmpC β-lactamase Resistance in Iran

Background & Objective: The production of plasmid-mediated AmpC beta-lactamases (PMABLs) among urinary Klebsiella pneumoniae isolates causes a severe problem to the successful treatment of urinary tract infections (UTIs). This study was designed to evaluate antimicrobial resistance, the presence of AmpC beta-lactamase genes, and the genetic relatedness among K. pneumoniae strains separated from...

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Inducible AmpC -Lactamase of a New Member Enterobacteriaceae

Extensive biochemical testing and 16S rRNA and rpoB sequence analysis revealed that clinical strain CF01Ent1, initially identified as Buttiauxella agrestis by the use of Api 32 biochemical strips, is a new organism in the Enterobacteriaceae family. It produced an inducible AmpC-type -lactamase whose sequence shares 69 to 72% identity with those of the other AmpC-type -lactamases of Enterobacter...

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ژورنال

عنوان ژورنال: Clinical Microbiology and Infection

سال: 2010

ISSN: 1198-743X

DOI: 10.1111/j.1469-0691.2009.02864.x