Prevalence, Antimicrobial Resistance, and Molecular Characteristics of Coagulase-Negative Staphylococci Isolated from Children’s Blood Cultures in Northeastern Iran Within 2013 - 2019

Background: Coagulase-negative staphylococci (CoNS) are rolled in severe infections animals and nosocomial humans. Given that other than Staphylococcus aureus often reported only as CoNS medical diagnosis laboratories, this study aimed to determine the exact species of type clinical samples. Objectives: This also evaluate antibiotic resistance, ability carry cfr, qacA/B, mecA, vanA genes, diversity staphylococcal cassette chromosome mec (SCCmec) elements mecA-carrying isolates. Methods: spp. strains were isolated from blood samples children admitted Imam Reza Hospital Bojnurd, Northeastern Iran, between 2013 - 2019. All isolates evaluated for resistance vancomycin oxacillin using agar screening routine anti-CoNS antibiotics Kirby-Bauer disk diffusion method, based on latest Clinical Laboratory Standards Institute guidelines. The conventional methods polymerase chain reaction (PCR)-restriction fragment length polymorphism. PCR was applied SCCmec Results: In study, 203 confirmed belonging nine S. capitis epidermidis top two common CoNS. Type III dominant mecA+ Conclusions: findings showed cultures have a relatively high resistance. Therefore, further attention should be paid isolation these they not easily considered contamination.