Preparative electrophoresis of human apolipoprotein E: an improved method.
نویسندگان
چکیده
منابع مشابه
Preparative electrophoresis: an improved method for the isolation of human recombinant apolipoprotein A-I.
Protein expression is a powerful technique to study the functional role of proteins both in vivo and in vitro. Various systems have been established to produce proteins in milligram quantities; however, the purification of many expressed proteins commonly results in trace amounts. We report on an improved method for the isolation of apolipoprotein A-I (apoA-I) using the Model 491 Prep Cell (Bio...
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A method of preparative electrophoresis is described. A volume of approximately 1 ml. of protein solution can be applied with the cell used. A “marker” solution, applied adjacent to the sample to be separated, indicates the positions of the protein bands during the migration. The procedure has the advantages of a high degree of recovery, simplicity of operation, and utilization of an electropho...
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Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system, with a complex etiology that includes a strong genetic component. The chromosome 19q 13 region surrounding the apolipoprotein E (APOE) gene has shown consistent evidence of involvement in MS. In a cross-sectional study, to show the APOE genotype and allele frequency in the MS population of Iran in comp...
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Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1990
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38745-9