Preparation of specific antisera against chicken lens protein components

Preparation of Type-specific Antisera to Reoviruses.

Preparation of type-specific antisera to reoviruses has been handicapped by the ubiquitous distribution of these viruses and frequent occurrence of reovirus antibodies in many species of animals. Antisera prepared in rhesus monkeys at this laboratory have shown extensive crossings between the three types (Table 1). Similar crossreactions have been observed in antisera prepared in rabbits (A. M....

Preparation, characterization, and localization of antisera against bovine MP26, an integral protein from lens fiber plasma membrane

Polyclonal antisera were prepared in rabbits using both native and chymotrypsin-digested bovine lens fiber plasma membranes. MP26, the principal protein of lens fiber plasma membranes, and CT20, a chymotryptic fragment of MP26, were isolated electrophoretically and used to purify anti-MP26 and anti-CT20 activity from the respective antisera by affinity chromatography. These affinity-purified an...

Studies on the Production of Specific Hyperimmune Antisera Against Type A F.M.D. Virus

Fast preparation of a polyclonal antibody against chicken protocadherin 1.

Protocadherins constitute a large family belonging to the cadherin superfamily; they function in various tissues of a wide variety of multicellular organisms. However, their functions and expression modes are still unknown in many of these species and tissues. We developed a fast and low-cost method to produce polyclonal antibody against chicken protocadherin 1 (Pcdh1) that could be used in ass...

Oocysticidal Effect of Essential Oil Components against Chicken Eimeria Oocysts

The objective of this study is to assess the ability of some major components of essential oils (EOs) to destroy Eimeria oocysts in vitro and to find out how the mechanism of action involved in this oocysticidal effect works. The methodology used was as follows: A screening of the oocysticidal activity of eight EO components was carried out in a liquid medium. The release of substances absorbin...