Preparation of a Cell-Free, Isobutene-Forming System from Rhodotorula minuta

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Preparation of a Cell-Free, Isobutene-Forming System from Rhodotorula minuta.

The cell-free isobutene-forming system of Rhodotorula minuta IFO 1102 was prepared. This system consisted of isovaleric acid, NADPH, and a cell extract. The maximal activity obtained by using the described cell-free system was 17 nl/mg of protein per h. It appears that this cell-free isobutene-forming system operates in living cells of R. minuta.

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Purification and characterization of cytochrome P450 from an isobutene-forming microorganism, Rhodotorula minuta.

A cytochrome P450 was purified from microsomes of Rhodotorula minuta. The optical spectrum of the purified cytochrome was characteristic of a low-spin ferric heme protein. Isovalerate caused a type I spectral change in it. The amino-terminal sequence of the cytochrome was different from those of other known microsomal cytochrome P450s. These results indicate that the cytochrome, which is tentat...

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Rhodotorula minuta fungemia in a ewe lamb.

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary D...

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Characterization and Safety Evaluation of Exopolysaccharide Produced by Rhodotorula minuta BIOTECH 2178

Yeast exopolysaccharide (EPS) are composed of unique sugar units, which offers an interesting perspective with respect to various food applications. This study aimed to characterize the EPS from Rhodotorula minuta BIOTECH 2178. Toxicological assessment of the EPS was investigated to ensure that it is safe for human consumption. The yeast EPS revealed high total sugar composition (59.2162-47%) a...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1988

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.54.2.583-584.1988