Preparation, Characterization, and Evaluation of Rutin Nanocrystals as an Anticancer Agent against Head and Neck Squamous Cell Carcinoma Cell Line

The reports show that rutin has good potentials as an anticancer agent; however, poor bioavailability due to its low aqueous solubility. present study was aimed at preparing and evaluating physicochemical properties well the activities of nanocrystals (RNs). RNs were prepared via ultrasonication method. then physicochemically characterized by conventional techniques. cytotoxic effect free on HN5 head neck squamous carcinoma cell line assessed. HGF1-PI1 cells normal oral treated RNs. Cells also exposed determine their effects expression caspase-8, caspase-9, Bcl-2, Bax genes. have a mean particle size 75 ± 0.16 nm quasispherical morphology. Rutin displayed no significant 2000 μM. However, with IC50 30.51 μM 27.34 in 24 48 h incubation times, respectively ( id="M2"> p < 0.05 ). had 100 times more than cells. There h. Bcl-2 mRNA significantly decreased attendance compared control group id="M3"> increase Bax/Bcl-2 ratio revealed within Our results confirm is rutin. activation mitochondria-dependent apoptotic pathway occurred modulation expression. These suggest may be useful development cancer therapy protocol.