Preparation and characterization of mouse IL-22 and its four single-amino-acid muteins that act as IL-22 receptor-1 antagonists

Preparation and characterization of mouse IL-22 and its four single-amino-acid muteins that act as IL-22 receptor-1 antagonists.

Recombinant mouse interleukin 22 (mIL-22) and its variants encoding four muteins (Y51A, N54A, R55A and E117A) were expressed in Escherichia coli, refolded and purified to homogeneity as monomeric proteins by one-step ion-exchange chromatography. The binding of IL-22 and its four muteins to immobilized mIL-22 receptor α1 extracellular domain (mIL-22 Rα1-ECD) exhibited similar affinity, indicatin...

IL 22 ( interleukin 22 )

IL-22 and IL-17: An Overview

Many studies from recent years have shown that cytokines like IL-22, IL-17A, and IL-17F play a major role in both the defense against certain microbes and the development and maintenance of chronic inflammatory diseases. These mediators are often secreted by subpopulations of T-helper cells called Th17 cells and Th22 cells, respectively. This chapter provides an overview about the common and di...

IL-22 vs. IL-22: The Tissue Matters

IL-22 is a cytokine recently discovered to be produced by TH17 cells. Its receptor is expressed in the skin, liver, the digestive and the respiratory tract where IL-22 conveys signals of the immune system directly to the tissue. IL-22 is involved in the host defense against extracellular pathogens as well as in autoimmune inflammatory diseases and exerts, depending on the tissue and inflammator...

Identification, cloning, and characterization of a novel soluble receptor that binds IL-22 and neutralizes its activity.

With the use of a partial sequence of the human genome, we identified a gene encoding a novel soluble receptor belonging to the class II cytokine receptor family. This gene is positioned on chromosome 6 in the vicinity of the IFNGR1 gene in a head-to-tail orientation. The gene consists of six exons and encodes a 231-aa protein with a 21-aa leader sequence. The secreted mature protein demonstrat...