Preliminary Analysis of Quality and Cryopreservation of Sex-Reversed Female Culter alburnus Sperm

Sex-reversed females are the fathers of all-female fish and their sperm quality directly affects fish. In this study, fecundity, physiological characteristics, biochemical ultrastructure, viability sex-reversed female after cryopreservation were investigated to understand quality. (1) The had a lower fertilization rate, hatching larval survival rate 72 hr higher deformation compared with normal male sperm. (2) changes in activation between under different salinity pH conditions same; optimum was 0 4%, 5.5. (3) preservation time is significantly shorter than that at room temperature. (4) ATPase content c/p value (5) Ultrastructural injury including damaged membranes head mitochondria, broken flagella, greater mitochondrial damage, membrane expansion found (6) most suitable antifreeze for 10% ethylene glycol dilution D-15, but movement, life span reduced cryopreservation. It speculated mitochondria more those male, which led differences fertility