Preformed mRNA in Cotyledons of Ungerminated Seeds of Cicer arietinum L.

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Preformed mRNA in Cotyledons of Ungerminated Seeds of Cicer arietinum L.

Polyadenylated RNA was isolated from total RNA extracted from cotyledons of ungerminated or 18-hour-germinated chick-pea seeds by affinity chromatography on oligo(dT)-cellulose. Both poly(A)-containing RNA fractions exhibited a template activity when assayed in two cell-free translation systems, wheat germ extracts, and nuclease-treated reticulocyte lysates. Translation of preformed mRNA from c...

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Understanding developmental processes, especially in non-model crop plants, is extremely important in order to unravel unique mechanisms regulating development. Chickpea (C. arietinum L.) seeds are especially valued for their high carbohydrate and protein content. Therefore, in order to elucidate the mechanisms underlying seed development in chickpea, deep sequencing of transcriptomes from four...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1980

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.65.6.1128