Predictive value of CK-MB test.

Limited diagnostic value of CK-MB.

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (mass), troponin and myoglobin measurements

Aim: The aim of the study was to investigate the effects of in vitro hemolysis on serum creatine kinase and creatine kinase MB isoenzyme activities with creatine kinase MB mass, troponin I and myoglobin measurements. Materials and methods: We prepared serum pools having analyte concentrations at normal and pathological values. Hemolysate was added into serum pools to obtain aliquotes with final...

Circulating CK-MB and CK-BB isoenzymes after prostate resection.

We studied the effect of prostate resection on serum creatine kinase (EC 2.7.3.2) and lactate dehydrogenase (EC 1.1.1.27) isoenzymes in 22 patients. Two hours after their operations, two-thirds of these patients had increased total creatine kinase activity. The MB isoenzyme was demonstrated in sera of 66% of the patients and the BB isoenzyme in 76%. MB content varied from 1 to 7% of total creat...

Circulating CK-MB and CK-BB isoenzyme after gastrointestinal surgery.

The effect of gastrointestinal surgery on serum creatine kinase activity was studied in 30 patients. The MB isoenzyme was demonstrated in sera of 30% of the patients and BB isoenzyme in 23%. MB content varied from 0.8 to 10.3% of the total creatine kinase activity, and the BB content from 0.6 to 18.4%. The CK-BB was probably of gastrointestinal origin, since gastrointestinal tract contains high...

Standardization of creatine kinase-MB (CK-MB) mass assays: the use of recombinant CK-MB as a reference material.

BACKGROUND The AACC assembled a committee to identify and validate a standard creatine kinase MB isoenzyme (CK-MB) material to improve the comparability of CK-MB mass assays. METHODS Three protocols were used. In protocol I, various CK-MB materials prepared in different matrices were screened as candidate standards. In protocol II, participating manufacturers calibrated their systems with con...