Prediction of Fetal Sex by Amplification of Fetal DNA Present in Cow Plasma
نویسندگان
چکیده
منابع مشابه
Prenatal determination of farm animal fetal sex using free fetal DNA in maternal plasma
In many farm animal species, the preference of a specific sex is obvious mainly due to commercial implications; so for comprehensive and efficient results in the livestock management plans specially breedingand selection programs, it is necessary to include acost-effective and reliable strategy for early determination of fetal sex. Several invasive and non-invasive approaches have been establis...
متن کاملA NEW WORKFLOW OF FETAL DNA PREDICTION FROM CELL-FREE DNA IN MATERNAL PLASMA by
Prediction of fetal DNA allows diagnosing known/passed mutations before child’s birth. Public health significance of such early testing is that it can reassure parents who have negative results and offers timely information for those with abnormal results. My dissertation work presents a new approach of reconstructing fetal DNA from maternal plasma. The method works because plasma from pregnant...
متن کاملEvaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma
OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...
متن کاملfetal sex determination using non-invasive method of cell-free fetal dna in maternal plasma of pregnant women during 6th– 10th weeks of gestation
in previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. with the discovery of cell-free fetal dna (cffdna) in plasma of pregnant women, access to...
متن کاملhighly sensitive conventional pcr testing of free fetal dna in maternal serum for non-invasive fetal sex determination
background: free fetal dna (ffd) in maternal plasma/serum has increasingly become the source of fetal material for diagnostic purposes in recent years. this source of fetal material can be used for sex determination. rh typing paternally inherited sequences and compound heterozygosity. reports on the lack of consistent pcr amplification of y-chromosome sequences of ffd in maternal plasma/serum ...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2010
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.10-010t