Predicting Sex-Related Transcripts in the Chinese Giant Salamander (Andrias davidianus): A Transcriptomics Study, Selection Gender for Preservation, Breeding and Reintroduction

The Chinese giant salamander (Andrias davidianus) is the biggest extant and endangered amphibian, which has an incongruous sex ratio during artificial breeding process indistinguishable in appearance. development mechanism of this rare species thus needs to be explored. We therefore conducted study explore molecular mechanisms hallmarks or gonad maintenance genes for CGS. In study, we sequenced analyzed sexually mature male female gonads salamanders (CGSs) using RNA-seq technology found a total 15,063 differentially expressed (DEGs) testicular ovarian tissues, 7973 (52.93%) were 7090 (47.07%) tissues. Thirteen DEGs selected based on gene expression abundance quantified muscle skin tissues both CGSs. results showed that these 13 DEGS, out 20 DEGs, only two them (Bmp15 ZP3) consistent compared CGSs sexes. Overall, our should provide important reference sex-characteristics-related type species, shedding new light future research topic.