Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection

This paper presents a potentiometric biosensor for the detection of amyloid β-42 (Aβ-42) in point-of-care analysis. approach is based on molecular imprint polymer (MIP) technique, which uses covalently immobilised Aβ-42 to create specific cavities surface single-walled carbon nanotubes (SWCNTs). The was prepared by binding SWCNT and then imprinting it adding acrylamide (monomer), N,N′-methylene-bis-acrylamide (crosslinker) ammonium persulphate (initiator). target peptide removed from matrix proteolytic action an enzyme (proteinase K). presence sites confirmed comparing MIP-modified with negative control (NIP) consisting similar material where molecule had been process. ability sensing rebind demonstrated incorporating MIP as electroactive compound PVC/plasticiser mixture applied solid conductive support graphite. All steps synthesis imprinted materials were followed Raman spectroscopy Fourier transform infrared (FTIR). analytical performance evaluated transduction, showed cationic slopes 75 mV-decade−1 buffer pH 8.0 limit 0.72 μg/mL. Overall, transduction that sensor can discriminate other biomolecules same solution.