Potential Translational Thioflavin T Methodology as a Complement of Cell-Based Assays and after Drug Exposition

Protein aggregation is a common characteristic of several human diseases such as Alzheimer’s disease. Recent evidence has indicated that the peptides p53 also marked in cancer cells. The aim this study was to correlate Thioflavin T (ThT) data with different cellular viability assays (Neutral Red and MTT) SH-SY5Y neuroblastoma cells HT-29 colon treated doxorubicin, classical antineoplastic agent. We studied effects well-known peptide Aβ42 on process these Our suggest both cell lines are responsive doxorubicin formed aggregates, highlighting relationship between ThT methodologies. observed lower values corresponded pronounced aggregation. Thus, results methodology used may complement assays. In addition, be interest evaluate role protein other