Potential of phage-displayed peptide library technology to identify functional targeting peptides
نویسندگان
چکیده
منابع مشابه
Development of antiangiogenin peptide using a phage-displayed peptide library.
Angiogenesis is essential for tumor growth and metastasis. Here, we have developed a peptide antagonist of human angiogenin, which is a potent and tumor-associated angiogenic factor. ANI-E peptide was derived from the phage clone, which binds to angiogenin via the disulfide-constrained octapeptide epitope that is displayed on its surface, and is displaced by actin. Disulfide-constrained ANI-E p...
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The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
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OBJECTIVE Vascular endothelial growth factor (VEGF) interaction with its receptor, VEGFR-3/Flt-4, regulates lymphangiogenesis. VEGFR-3/Flt-4 expression in cancer cells has been correlated with clinical stage, lymph node metastasis, and lymphatic invasion. The objective of this study is to identify a VEGFR-3/Flt-4-interacting peptide that could be used to inhibit VEGFR-3 for ovarian cancer thera...
متن کاملTrinucleotide cassettes increase diversity of T7 phage-displayed peptide library
BACKGROUND Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and showed that T7 phage can display a more diverse amino acid sequence repertoire due to differing processes of viral morphogenesis. METHODS In t...
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Experimental section General All chemicals and solvents for peptide synthesis were of reagent or HPLC grade and were used without further purification. TARON resin was purchased from Clontech. Dihydrofolate (DHP), nicotinamide adenine dinucleotide phosphate (NADPH) and methotrexate (MTX) were purchased from Wako Pure Chemical Industries. Biotin-AC5-OSu and 2-morpholinoethanesulfonic acid monohy...
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ژورنال
عنوان ژورنال: Expert Opinion on Drug Discovery
سال: 2007
ISSN: 1746-0441,1746-045X
DOI: 10.1517/17460441.2.4.525