Potential Impacts of Clove Essential Oil Nanoemulsion as Bio Fungicides against Neoscytalidium Blight Disease of Carum carvi L.

Fungal plant diseases are considered one of the most destructive for plants. In this current study, Neoscytalidium dimidiatum as a fungal pathogen was isolated from an infected Carum carvi L. (C. carvi) first time and controlled it using clove essential oil nanoemulsion (CEONE). This identified morphologically genetically, then deposited in gene bank under accession number OQ338188. CEONE prepared characterized DLS, Zeta potential, TEM analysis. The characterization results illustrated that size lower than 100 nm, which confirmed by TEM, appeared spherical shape. Results revealed has promising antifungal activity towards N. dimidiatum, where inhibition percentages at concentrations 5000, 3000, 1000 ppm were 82.2%, 53.3%, 25.5%, respectively. At pot level, highly virulent on C. carvi, CEO effective against blight disease, with 71.42% 57.14% protection, Moreover, there vast improvement root length, height, leaves. Additionally, application induced resistance modifying proline, phenol, hydrogen peroxide, malondialdehyde, antioxidant enzymes. conclusion, but can be effectively reduced through eco-friendly method.