Potensi Aktivitas Bakteriosin Lactobacillus Gasseri Terhadap Pertumbuhan Salmonella Typhi

نویسندگان

چکیده

Latar belakang. Lactobacillus gasseri merupakan jenis bakteri asam laktat (BAL) yang dapat menghasilkan bakteriosin sebagai suatu senyawa antibakteri. Objektif. untuk mengetahui potensi aktivitas terhadap pertumbuhan Salmonella typhi dengan menilai Kadar Hambat Minimal (KHM) dan Bunuh (KBM). Metode. Penelitian ini dilakukan teknik dilusi menggunakan desain Rancangan Acak Lengkap. Konsentrasi dibagi dalam berbagai kelompok konsentrasi yaitu 100%, 80%, 60%, 40%, 20%, 10% 0% (kontrol) empat kali pengulangan. Nilai KHM KBM ditentukan menghitung jumlah koloni tumbuh dari masing-masing konsentrasi. Hasil penelitian dianalisis secara statistik uji One-way ANOVA Post-Hoc LSD. Hasil. menunjukkan bahwa menghambat namun tidak ditemukan nilai KBM. Dengan perhitungan perbedaan bermakna pada 40% 20% kontrol. Simpulan. memiliki efek bakteriostatik tetapi bakterisidal. Kemampuan daya hambat dipengaruhi oleh bakteriosinya.
 Kata kunci: Antibakteri, Bakteriosin, gasseri, typhi
 
 Background. is a type of lactic acid bacteria that can produce bacteriocin as an antibacterial compound. Objective. To determine the potential activity on growth by assessing Minimum Inhibitory Level (MIC) and Bactericidal Concentration (MBC). Method. This research was conducted with dilution technique using completely randomized design. The concentration divided into various groups namely (control) four repetitions. MIC MBC values were determined counting number bacterial colonies grew from each concentration. results study statistically analyzed LSD tests. Results. showed could inhibit but found no values. Statistical calculations significant differences in controls. Conclusion. has bacteriostatic effect bactericidal effect. ability to influenced bacteriocytes.
 Keyword: Antibacterial, Bacteriocin,

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ژورنال

عنوان ژورنال: Jurnal Ilmu Kesehatan Indonesia

سال: 2021

ISSN: ['2722-4848']

DOI: https://doi.org/10.25077/jikesi.v1i3.80