Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine.
نویسندگان
چکیده
منابع مشابه
Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection.
A whole-column detection (WCD) system was fabricated using the parts from a typical A4 size optical scanner. Optimum layout of the fluorescence tube, beam condenser and photon transducers from the scanner made an effective devise to examine the dynamic chromatographic separation process. This system was applied in monitoring peak crossover in an ion-pair chromatographic elution. A stage-wise gr...
متن کاملAnalysis of corticosteroids in biological fluids by high-performance liquid chromatography with fluorometric detection.
strong sulfuric acid solutions, and can be measured by a fluorometer.1) Gotelli et al.2) and Shihabi et al.3) have used high-performance liquid chromatography (HPLC) to separate fluorescent derivatives, and have measured cortisol levels in serum and urine. Since the sulfuric acid-induced fluorescence is unstable, it is necessary to inject the reaction mixture into HPLC immediately after the for...
متن کاملDetection of Oxytetracycline Residue in Infant Formula by High-Performance Liquid Chromatography (HPLC)
Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...
متن کاملA Novel High-Performance Liquid Chromatography Method for Detection of Alginate in Pseudomonas aeruginosa
Background and Objective: The opportunistic pathogen Pseudomonas aeruginosa secrets a capsule-like polysaccharide called alginate which is important for evasion of host defenses, especially in patients with suppressed immunity. Method of alginate determination has an important role in the study of microb...
متن کاملDetermination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection
Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1983
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.32.6_e207