Porins of Brucella species
نویسندگان
چکیده
منابع مشابه
Molecular characterisation of Brucella species.
The genus Brucella (Mayer and Shaw, 1920) currently consists often species with validly published names. Within most species further differentiation into biovars exists. Genetically, all Brucella species are highly related to each other, exhibiting sequence similarity values of 98% to 100% in aligned regions (core genome). The population structure is clonal. Despite this close genetic relatedne...
متن کاملIn vitro mutagenesis of Brucella species.
Three major techniques have been employed for broad-range in vitro mutagenesis of Brucella species. Shotgun approaches capable of generating large libraries of randomly inserted transposon mutants include Tn5, mariner (Himar1), and mini-Tn5 signature-tagged mutagenesis. Allelic exchange has also been extensively employed for targeted gene deletion. In general, plasmid and transposon delivery in...
متن کاملPhage typing reactions on Brucella species.
The nature of the phage typing reactions on Brucella species was determined by rates of adsorption and infection, one-step growth experiments, and susceptibility to lysis from without. The highest rates of adsorption and infection were obtained on smooth B. abortus cultures, and large clear plaques were produced. One or a few phage particles per B. neotomae cell killed about one-half of the cel...
متن کاملImproved expression vector for Brucella species.
We constructed an improved vector pNSGroE for gene expression studies in Brucella spp. It is derived from the broad host range cloning vector pBBR1MCS (1). This new plasmid has several advantages over pBBR1MCS or its derivatives pBBGroE (2): (i) it is smaller in size, 2.9 kb; (ii) it expresses proteins as His-tagged fusion for easy detection and purification; (iii) it carries the groE promoter ...
متن کاملPolymorphism at the dnaK locus of Brucella species and identification of a Brucella melitensis species-specific marker.
The dnaK gene and surrounding sequences from reference strains of the six Brucella species were amplified by the polymerase chain reaction (PCR) with primers chosen according to the published sequence of the B. ovis dnaK gene and studied for polymorphism with nine restriction endonucleases. The restriction patterns were identical for all species with all restriction endonucleases tested except ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1984
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.44.1.16-21.1984