Porcine endogenous retrovirus infection of human peripheral blood mononuclear cells
نویسندگان
چکیده
منابع مشابه
Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...
متن کاملType C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells.
As part of the evaluation of porcine cells, tissues, and organs intended for transplantation into humans, we investigated the conditions required to induce expression and release of porcine endogenous retrovirus (PoEV) from primary cells. Pigs contain endogenous retroviral sequences encoding infectious retrovirus, yet little is known about the conditions required to activate the expression and ...
متن کاملInfection of Peripheral Blood Mononuclear Cells
Infection by herpesviruses can result in profound immunosuppressive or immunomodulatory effects. However, no significant information is available on the effect of such infections on the production of immunoregulatory cytokines. We studied the kinetics of production of two monocyte-derived cytokines, interleukin 6 (IL-6) and tumor necrosis factor-a (TNFa), induced by Epstein-Barr virus (EBV) and...
متن کاملSusceptibility of human liver cells to porcine endogenous retrovirus.
OBJECTIVES The risk of porcine endogenous retrovirus infection is a major barrier for pig-to-human xenotransplant. Porcine endogenous retrovirus, present in porcine cells, can infect many human and nonhuman primate cells in vitro, but there is no evidence available about in vitro infection of human liver cells. We investigated the susceptibility of different human liver cells to porcine endogen...
متن کاملoptimization of human peripheral blood mononuclear cells (pbmcs) cryopreservation
cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...
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ژورنال
عنوان ژورنال: Xenotransplantation
سال: 2014
ISSN: 0908-665X
DOI: 10.1111/xen.12150