Porcine dentin sialoprotein glycosylation and glycosaminoglycan attachments
نویسندگان
چکیده
منابع مشابه
Characterization of porcine dentin sialoprotein (DSP) and dentin sialophosphoprotein (DSPP) cDNA clones.
Dentin sialophosphoprotein (DSPP) is a chimeric glycoprotein with dentin sialoprotein (DSP) on its N-terminus and dentin phosphoprotein (DPP) on its C-terminus. We have constructed and screened a unidirectional cDNA library derived from the pulp organ of developing pig teeth, and isolated cDNA clones encoding DSP-only, as well as two DSPP clones with alternative sequences in their 3' coding reg...
متن کاملEstablishment of porcine pulp-derived cell lines and expression of recombinant dentin sialoprotein and recombinant dentin matrix protein-1.
The major non-collagenous proteins in dentin have extensive post-translational modifications (PTMs) that appear to be odontoblast-specific, so expression of recombinant dentin proteins in other cell types does not achieve the in vivo pattern of PTMs. We established cell lines from developing porcine dental papillae and used them to express recombinant dentin sialoprotein (DSP) and dentin matrix...
متن کاملA novel rat dentin mRNA coding only for dentin sialoprotein.
Dentin sialoprotein (DSP) is a major glycoprotein present in the mineralized dentin matrix that is expressed mainly by young and mature odontoblasts. Mutations in the DSP coding regions are linked to Dentinogenesis imperfecta I and II. indicating the importance of DSP in tooth formation. Previous studies have identified multiple mRNA transcripts in dentin that code for both DSP and phosphophory...
متن کاملTensile characterization of porcine temporomandibular joint disc attachments.
The frequency and impact of temporomandibular joint (TMJ) disorders necessitate research in characterizing the joint's function. The 6 discal attachments have not yet been systematically characterized under tension. Understanding their role in joint function may guide our study of TMJ pathologies, including disc displacement. In the present study, a porcine model was used to characterize the at...
متن کاملGenomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein (Dspp) gene, which codes for both dentin sialoprotein and dentin phosphoprotein.
Our laboratory has reported that two major noncollagenous dentin proteins, dentin sialoprotein and dentin phosphoprotein, are specific cleavage products of a larger precursor protein termed dentin sialophosphoprotein (MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J. Q., and Gu, T. T. (1997) J. Biol. Chem. 272:835-842). To confirm our single gene hypothesis and initiate in vitro pro...
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ژورنال
عنوان ژورنال: BMC Biochemistry
سال: 2011
ISSN: 1471-2091
DOI: 10.1186/1471-2091-12-6