Polymerase chain reactions with alphoid-repeat primers in combination with Alu or LINEs primers, generate chromosome-specific DNA fragments

Polymerase chain reactions with alphoid-repeat primers in combination with Alu or LINEs primers, generate chromosome-specific DNA fragments.

Y alphoid primers in combination with Alu and LINEs primers generated new DNA fragments in polymerase chain reactions (PCR) on DNA from a Y-only somatic cell hybrid but not from X-only, 3-only, or 21-only hybrids. X alphoid primers used in a similar manner generated new DNA fragments from the X-only hybrid, and 1 of the primers (X2) also generated new DNA fragments on 3-only and 21-only hybrids...

Eliminating primers from completed polymerase chain reactions with exonuclease VII.

Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR product are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Species-specific Polymerase Chain Reaction Primers for Detecting Prevotella nigrescens

A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop Prevotella nigrescens-specific polymerase chain reaction (PCR) primers. The specificity was tested against 10...

Detection and Differentiation of Phaeoisariopsis griseola Isolates with the Polymerase Chain Reaction and Group-Specific Primers

Angular leaf spot (ALS) disease of common bean (Phaseolus vulgaris L.) is caused by the imperfect fungus Phaeoisariopsis griseola (Sacc.) Ferraris. ALS is an important disease in tropical and subtropical countries and can cause severe bean yield losses (approximately 40 to 80%) under favorable environmental conditions (5,7,19,20). Breeding for disease resistance is the most practical approach f...