Poly‐L‐lysine amplification of protamine immobilization and heparin adsorption
نویسندگان
چکیده
منابع مشابه
Poly-L-lysine amplification of protamine immobilization and heparin adsorption.
We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method t...
متن کاملProtamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane.
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The current study examined the effects of variables on the immobilization of protamine to cyanogen bromide (CNBr)-activated cellulose hollow fibers. The degree of protamine immobiliza...
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Heparin, an anticoagulant, has been used in many forms to treat various diseases. These forms include soluble heparin and heparin immobilized to supporting matrices by physical adsorption, by covalent chemical methods and by photochemical attachment. These immobilization methods often require the use of spacers or linkers. This review examines and compares various techniques that have been used...
متن کاملHigh incidence of antibodies to protamine and protamine/heparin complexes in patients undergoing cardiopulmonary bypass.
Protamine is routinely used to reverse heparin anticoagulation during cardiopulmonary bypass (CPB). Heparin interacts with protamine to form ultralarge complexes that are immunogenic in mice. We hypothesized that patients exposed to protamine and heparin during CPB will develop antibodies (Abs) to protamine/heparin (PRT/H) complexes that are capable of platelet activation. Specimens from a rece...
متن کاملHemolysis and alimentary lipemia; effects of incubation, heparin, and protamine.
ITTLE ATTENTION has been paid to the observation of Longimui and Johnsontm that an alimentary lipemia may be responsible for increased fragility of erythrocytes. The possibility that the hemolysis may also occur in vivo is suggested by Loewy, et al.,2 who demonstrated that the output of bile pigment increased in dogs receiving high fat diets. More recently, Creditor3 has observed that hemolysis...
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ژورنال
عنوان ژورنال: Journal of Biomedical Materials Research
سال: 1998
ISSN: 0021-9304,1097-4636
DOI: 10.1002/(sici)1097-4636(199811)42:2<182::aid-jbm3>3.3.co;2-s