Polyketide Synthase Gene Responsible for Citrinin Biosynthesis in Monascus purpureus
نویسندگان
چکیده
منابع مشابه
Exploring the Distribution of Citrinin Biosynthesis Related Genes among <named-content content-type="genus-species" type="simple">Monascus</named-content> Species
Citrinin, a hepato-nephrotoxic compound to humans, can be produced by the food fermentation microorganisms Monascus spp. In this study, we investigated the distribution of mycotoxin citrinin biosynthesis genes in 18 Monascus strains. The results show that the acyl-transferase and ketosynthase domains of the pksCT gene encoding citrinin polyketide synthase were found in Monascus purpureus, Monas...
متن کاملIdentification and in vivo functional analysis by gene disruption of ctnA, an activator gene involved in citrinin biosynthesis in Monascus purpureus.
Citrinin, a secondary fungal metabolite of polyketide origin, is moderately nephrotoxic to vertebrates, including humans. From the red-pigment producer Monascus purpureus, a 21-kbp region flanking pksCT, which encodes citrinin polyketide synthase, was cloned. Four open reading frames (ORFs) (orf1, orf2, orf3, and orf4) in the 5'-flanking region and one ORF (orf5) in the 3'-flanking region were ...
متن کاملPigments and citrinin biosynthesis by fungi belonging to genus Monascus.
Citrinin is a mycotoxin, which is produced by fungi belonging to the genus Monascus, known in biotechnology as producers of azaphilone pigments. The relation between biosynthesis of these secondary metabolites was investigated in different species of the genus Monascus in batch-culture at the following cultivation conditions: T = 28 degrees C, agitation 220 rpm, and a medium, which induce citri...
متن کاملEFFECT OF DISSOLVED OXYGEN CONCENTRATION ON RED PIGMENT AND CITRININ PRODUCTION BY Monascus purpureus ATCC 36928
The present study investigated the effects of agitation speed, N (200, 500, 600 or 700 rpm), and dissolved oxygen concentration, C (120, >70, 70, 60, 10 or 10%), on red pigment and citrinin production by Monascus purpureus ATCC 36928, cultivated in liquid medium by a batch process. The gas flow rate was the same for all runs with C controlled by means of the incoming gas composition control (ai...
متن کاملConstruction of a replacement vector to disrupt pksCT gene for the mycotoxin citrinin biosynthesis in Monascus aurantiacus and maintain food red pigment production.
More and more people pay attention to citrinin produced by Monascus, which has nephrotoxic activity in mammals. It was reported that pksCT gene is responsible for citrinin biosynthesis in Monascus purpureus. In this paper, two DNA fragments in both ends of pksCT were amplified by genomic PCR from fourteen Monascus spp. strains. The PCR products were gained from all of the strains. It is suggest...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2005
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.71.7.3453-3457.2005