Polyketide Synthase Engineering Toward Improved Antibiotics
نویسندگان
چکیده
منابع مشابه
Recombinant polyketide synthesis in Streptomyces: engineering of improved host strains.
Efficient polyketide synthesis derived from plasmid-borne heterologous Streptomyces polyketide synthase (PKS) gene clusters necessitates a suitable host strain. Well-characterized laboratory strains such as Streptomyces coelicolor or Streptomyces lividans and their frequently used derivatives carry endogenous genes for the synthesis of actinorhodin (among other PKS genes), which might interfere...
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Cyanobacteria are considered a promising source for new pharmaceutical lead compounds and a large number of chemically diverse and bioactive metabolites have been obtained from cyanobacteria. Despite of several worldwide studies on prevalence of NRPSs and PKSs among the cyanobacteria, none of them included Iranian cyanobacteria of Kermanshah province. Therefore, the aim of this study was t...
متن کاملNovel polyketide synthase from Nectria haematococca.
We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase, duplicated acyl carrier proteins, and thioesterase. The pksN product groups ...
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Polyketides (PKs) are a class of natural products (secondary metabolites) from bacteria, fungi, and plants, including many clinically important drugs such as tetracycline, daunorubicin, erythromycin, rapamycin and lovastatin [1, 4]. They have a large diversity of biological activities and pharmacological properties such as antibiotic, anticancer, antifungal, antiparasitic, cytostatic, and immun...
متن کاملSubstrate Controlled Divergence in Polyketide Synthase Catalysis
Biochemical characterization of polyketide synthases (PKSs) has relied on synthetic substrates functionalized as electrophilic esters to acylate the enzyme and initiate the catalytic cycle. In these efforts, N-acetylcysteamine thioesters have typically been employed for in vitro studies of full PKS modules as well as excised domains. However, substrate engineering approaches to control the cata...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.lb300