Polyionic and cysteine-containing fusion peptides as versatile protein tags
نویسندگان
چکیده
منابع مشابه
Polyionic and cysteine-containing fusion peptides as versatile protein tags.
In response to advances in proteomics research and the use of proteins in medical and biotechnological applications, recombinant protein production and the design of specific protein characteristics and functions has become a widely used technology. In this context, protein fusion tags have been developed as indispensable tools for protein expression, purification, and the design of functionali...
متن کاملAlkylation of cysteine-containing peptides to mimic palmitoylation.
Numerous proteins that are involved in cell signaling and viral replication require post-translational modification by palmitoylation to function properly. The molecular details by which this palmitoyl modification affects protein function remain poorly understood. To facilitate in vitro biochemical and structural studies of the role of palmitoylation on protein function, a method was developed...
متن کاملProtein disulphide isomerase-mediated grafting of cysteine-containing peptides onto over-bleached hair
The ability of Protein disulphide isomerase (PDI) to promote the grafting of two cysteine-containing peptides onto hair was investigated in order to develop an alternative treatment for over-bleached hair. The studied peptides were designed based on human keratin and human lung surfactant proteins and were linked to a fl uorescent dye to facilitate visualisation of the grafting process and to a...
متن کاملOn-line counting of cysteine residues in peptides during electrospray ionization by electrogenerated tags and their application to protein identification.
The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone compounds on an undecapeptide containing one cysteine residue. 2-carboxymethylhydroquinone was the most reactive probe and revealed to be suitab...
متن کاملOrg . On Cysteine and Cystine Peptides . Part V . l S - Trityl - and S - Diphenyl - methyl - cysteine and - cysteine Peptides
A new method for the preparation of S-trityl(i.e. triphenylmethyl) and S-diphenylmethyl-cysteines or -cysteine peptides involves treatment of cysteine or cysteine peptides with aralkylcarbinols in the presence of trifluoroacetic acid or hydrogen halides in acetic acid solution. The optimal conditions for the removal of these S-protecting aralkyl groups by trifluoroacetic acid or hydrogen halide...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological Chemistry
سال: 2013
ISSN: 1437-4315,1431-6730
DOI: 10.1515/hsz-2013-0116