Polyhydroxyalkanoates (PHAs) Production from Residual Glycerol by Wild Type Cupriavidus necator

The main objective of the present study is to assess and improve fermentation conditions produce polyhydroxyalkanoates (PHAs), such as homopolymer poly(3-hydroxybutyrate) (PHB) copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), from renewable inexpensive industrial waste by wild type Cupriavidus necator. Untreated residual glycerol, a byproduct generated biodiesel industry, was used carbon source C. bacterial adaptation stage, glycerol concentration other medium components were adjusted enhance PHB production. Fed batch with pulse feeding strategy propionic acid solution employed for PHBV necator, previously adapted utilization, able accumulate up 4.1 g/l under optimal substrate conditions. bacterium could also synthesize 3.4 PHBV, mole fraction 3-hydroxyvalerate (3HV) 12% fed-batch pulsed feed strategy. This first report on use ideal feedstock PHA production necator strain.