Polyclonal hyper-IgE mouse model reveals mechanistic insights into antibody class switch recombination

Polyclonal hyper-IgE mouse model reveals mechanistic insights into antibody class switch recombination.

Preceding antibody constant regions are switch (S) regions varying in length and repeat density that are targets of activation-induced cytidine deaminase. We asked how participating S regions influence each other to orchestrate rearrangements at the IgH locus by engineering mice in which the weakest S region, Sε, is replaced with prominent recombination hotspot Sμ. These mice produce copious po...

Molecular Mechanisms of IgE Class Switch Recombination.

Immunoglobulin (Ig) E is the most tightly regulated of all Ig heavy chain (IgH) isotypes and plays a key role in atopic disease. The gene encoding for IgH in mature B cells consists of a variable region exon-assembled from component gene segments via V(D)J recombination during early B cell development-upstream of a set of IgH constant region CH exons. Upon activation by antigen in peripheral ly...

POLYCLONAL IgE CLASS - RESTRICTED TOLERANCE

Antibody responses of the immunoglobulin E (IgE) class are regulated by a finely tuned network of cellular and molecular interactions. The differentiation of IgE B lymphocytes is controlled by antigen-specific and non-antigen-specific T helper and suppressor cells and their molecular products (1-3). The antigen-specific cellular interaction can be carried out by thymus-derived (T) cells which r...

Related Mechanisms of Antibody Somatic Hypermutation and Class Switch Recombination.

The primary antibody repertoire is generated by mechanisms involving the assembly of the exons that encode the antigen-binding variable regions of immunoglobulin heavy (IgH) and light (IgL) chains during the early development of B lymphocytes. After antigen-dependent activation, mature B lymphocytes can further alter their IgH and IgL variable region exons by the process of somatic hypermutatio...

Interallelic Class Switch Recombination