Poly(C)-binding protein interacts with the hepatitis C virus 5' untranslated region.

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Poly(C)-binding protein interacts with the hepatitis C virus 5' untranslated region.

We have investigated whether poly(C)-binding protein (PCBP)-1 and PCBP-2 interact with the hepatitis C virus (HCV) 5' untranslated region. Our results demonstrate that glutathione S-transferase (GST)-PCBP-1 and GST-PCBP-2 fusion proteins bind specifically to the HCV 5' untranslated region. An antiserum raised against PCBP-2 induced a supershift after incubation with RNA-protein complexes formed...

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PolyC-Binding Protein 1 Interacts with 5′-Untranslated Region of Enterovirus 71 RNA in Membrane-Associated Complex to Facilitate Viral Replication

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Hepatitis C Virus 39X Region Interacts with Human Ribosomal Proteins

To identify proteins that can bind the 3* untranslated region (UTR) of hepatitis C virus (HCV) we screened human cDNA libraries using the Saccharomyces cerevisiae three-hybrid system. Screening with an RNA sequence derived from the 3*-terminal 98 nucleotides (3*X region) of an infectious clone of HCV (H77c) yielded clones of human ribosomal proteins L22, L3, S3, and mL3, a mitochondrial homolog...

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Direct detection of circulating hepatitis C virus RNA using probes from the 5' untranslated region.

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It is unclear whether the sequence populations of hepatitis C virus (HCV) quasispecies in the liver and in serum are different, as a variety of studies on this subject provide conflicting results. In the current study, the populations of HCV 5' untranslated region (5' UTR) sequences in paired serum and liver samples from six patients with chronic hepatitis were analysed. Liver-derived, negative...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1999

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-80-6-1371