Polyacrylamide-based phantoms of human skin for hyperspectral fluorescence imaging and spectroscopy

Abstract Based on the combined use of polymerisable polyacrylamide, collagen and an aqueous solution Flavin adenine dinucleotide (FAD), we have developed a new technique for fabrication composite phantoms mimicking fluorescence properties human skin. A comparative analysis absorption scattering coefficients, refractive indices, as well spectra with different concentrations FAD measured CCD spectrometer hyperspectral camera is presented. To obtain values coefficients close to those skin, zinc oxide (ZnO) nanoparticles are added polyacrylamide polymer structure. Variations both in shape spectrum intensity signal provided by changes volume fraction collagen. It shown that model good agreement results direct skin measurements vivo .