Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes

Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.

We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times that the technique can analyze. In particular, we examine various methods to ana...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Fluorescence correlation spectroscopy.

Dna Condensation Characterised by Fluorescence Correlation Spectroscopy (fcs)

MARTIN HOF, TERESA KRAL, MAREK LANGNER, NOPPADON ADJIMATERA and IAN S. BLAGBROUGH J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, CZ-18223 Prague, Czech Republic, Agricultural University, Department of Physics and Biophysics, Wrocław, Poland, Wrocław University of Technology, Wrocław, Poland, Academic Centre for Biotechnology of Lipids Aggr...

Multiphoton Fluorescence Correlation Spectroscopy (MPFCS)

Nanoparticles have been demonstrated as a promising new generation of specific imaging agents and targeted delivery vehicles for the diagnosis and treatment of solid tumors. Nonetheless, for optimal therapy of tumors, the nanoparticles must penetrate the tumor interstitial matrix to reach the cancer cells. This thesis describes the development of a multiphoton fluorescence correlation spectrosc...