Plumbagin production by root cultures of Plumbago rosea

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Plumbagin production by root cultures of Plumbago rosea

∗ Corresponding author Root cultures of Plumbago rosea Linn. were established from young leaf explants on solid Gamborg's B5 (B5) medium supplemented with the combination of αnaphthalene acetic acid (NAA) and kinetin in the concentration ranges of 0.5-2.0 mg/l and 0.1-0.5 mg/l, respectively. The production of plumbagin, determined by TLC-densitometry was higher [0.016 ± 0.0030% dry weight (DW)]...

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Antibacterial Activity of Plumbagin and Root Extracts of Plumbago Zeylanica

Over the past few decades there has been much interest in natural materials as sources of new antibacterial agents. Different extracts from traditional medicinal plants have been tested. Many reports show the effectiveness of traditional herbs against microorganisms; as a result, plants have become one of the bases of modern medicine (Evans et al., 2002). Plants have given the Western pharmacop...

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Isolation of morphovariants through plant regeneration in Agrobacterium rhizogenes induced hairy root cultures of Plumbago rosea L

In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...

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Cell Suspension Culture of Plumbago europaea L. Towards Production of Plumbagin

Background: Plumbagin is as an important bioactive secondary metabolite found in the roots of Plumbago spp. The only one species, Plumbago europaea L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots. O...

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Antimicrobial activity in vitro of plumbagin isolated from Plumbago species.

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ژورنال

عنوان ژورنال: Electronic Journal of Biotechnology

سال: 2002

ISSN: 0717-3458,0717-3458

DOI: 10.2225/vol5-issue3-fulltext-4