Platelet resistance to prostaglandin D2 in patients with myeloproliferative disorders

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Platelet resistance to prostaglandin D2 in patients with myeloproliferative disorders.

We investigated the effect of prostaglandin 03 (PGD2) on platelet adenylate cyclase activity in 23 patients with myeloproliferative disorders, including eight patients with polycythemia vera, seven with myeloid metaplasia, four with chronic myelogenous leukemia. and four with essential thrombocythemia. In 20 of these patients there was less activation of platelet adenylate cyclase at all concen...

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Characterization of the platelet prostaglandin D2 receptor. Loss of prostaglandin D2 receptors in platelets of patients with myeloproliferative disorders.

Prostaglandin (PG) D(2) is synthesized in platelets at concentrations which could inhibit aggregation via activation of adenylate cyclase. To more directly define platelet-PG interactions, a binding assay has been developed for platelet PG receptors with [(3)H]PGD(2) as ligand. [(3)H]PGD(2) binding to intact platelets was saturable and rapid with the ligand bound by 3 min at 20 degrees C. PG co...

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Increased platelet-fibrinogen affinity in patients with myeloproliferative disorders.

Patients with myeloproliferative disorders (MPD) are known to have some abnormalities of platelet glycoproteins (Gp). Quantitative changes of the Gp Ib, IIb-IIIa, and/or their glucidic content have been reported. Since the Gp IIb-IIIa complex plays a major role in fibrinogen binding by activated platelets, we measured the platelet fibrinogen affinity in nine patients with polycythemia vera (PV)...

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Platelet-derived growth factor concentrations in platelet-poor plasma and urine from patients with myeloproliferative disorders.

Our enzyme-linked immunosorbent assay (ELISA) for measuring human platelet-derived growth factor (PDGF) detects nanogram quantities (ranging from 0.007 to 16 ng/100 microL) in purified PDGF standards. This assay is sensitive enough for studying plasma and urine. The range in normal volunteers was 0.6 to 2.3 micrograms/L for platelet-poor plasma and 1.4 to 3.3 micrograms/L for urine. We determin...

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Low Frequency of C-MPL Gene Mutations in Iranian Patients with Philadelphia-Negative Myeloproliferative Disorders

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ژورنال

عنوان ژورنال: Blood

سال: 1978

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v52.3.618.618