Plasmid transformation in Haemophilus influenzae
نویسندگان
چکیده
منابع مشابه
Plasmid transformation in Haemophilus influenzae.
Purified 34-megadalton-plasmid deoxyribonucleic acid from antibiotic-resistant strains of Haemophilus influenzae transforms competent strains of H. influenzae more efficiently if the recipient strains contain certain other 30-megadalton plasmids.
متن کاملPlasmid establishment in competent Haemophilus influenzae occurs by illegitimate transformation.
Establishment of plasmids in naturally competent Haemophilus influenzae is incompatible with transformation via the normal DNA translocation pathways. Instead, establishing plasmids appear to evade the degradation which ordinarily accompanies translocation, arriving as intact double-stranded molecules in the cytoplasm. Evidence is presented that plasmid establishment is a rare illegitimate tran...
متن کاملPlasmid-to-plasmid recombination in Haemophilus influenzae.
No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.
متن کاملTransformation of Haemophilus influenzae.
Conjugation: Although H. influenzae genes may be transferred by plasmid-mediated conjugation, this process is not discussed further below as it has received little attention and is rarely used for strain construction. Conjugative plasmids are common in H. influenzae as in other bacteria [1, 2, 3]. The F plasmid of Escherichia coli can conjugate into H. influenzae cells [4] (and into many other ...
متن کاملPlasmid-mediated chloramphenicol resistance in Haemophilus influenzae.
A clinical isolate of Haemophilus influenzae HC234 was found to be resistant to chloramphenicol and tetracycline. It was shown that both resistance markers are tranferable as one unit to other Haemophilus influenzae strains and also to Escherichia coli. Data are presented which indicate that conjugation is the most likely mechanism of resistance transfer. HC234 was shown to carry a single plasm...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.145.2.1099-1101.1981