Plasmid Curing of <i>Escherichia coli</i> Cells with Ethidium Bromide, Sodium Dodecyl Sulfate and Acridine Orange
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چکیده
منابع مشابه
Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which w...
متن کاملStability and structural features of DNA intercalation with ethidium bromide, acridine orange and methylene blue
Ethidium bromide (EB), acridine orange (AO), methylene blue (MB) and other fluorescent compounds are often used to probe DNA structure in drug–DNA and protein–DNA interactions. They bind nucleic acids via intercalative mode and cause major changes to DNA and RNA structures. The aim of this study was to examine the stability and structural features of calf-thymus DNA complexes with EB, AO andMB ...
متن کاملIn situ analysis of apoptosis in Aspergillus nidulans with ethidium bromide and acridine orange.
Apoptosis and necrosis are among several types of cell death. We stained the nuclei of Aspergillus nidulans grown in micro-colonies with ethidium bromide and acridine orange to detect in situ apoptosis. Suspensions of conidia from 5-day-old colonies of the A. nidulans strains biA1methG1, G422, CLC100, and CLB3 were each put into two tubes. The suspension of one tube was irradiated with ultravio...
متن کاملStudy of Peculiarities of Differential Absorption Spectra of Complexes of Acridine Orange, Ethidium Bromide and Methylene Blue with Dna
Interaction of intercalators: ethidium bromide (EtBr), methylene blue (MB) and acridine orange (AO), with DNA at 0.02 M ionic strength of the solution has been studied by differential spectroscopy method. It was revealed that EtBr binds to DNA by intercalation, semi-intercalation and electrostatic modes; MB by semiintercalation and electrostatic modes and AO by intercalation and electrostatic m...
متن کاملLoss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.
Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of curing by SDS with the curing effects exerted by elevated temperature and ethid...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Microbiology
سال: 1970
ISSN: 2408-8374,1011-9981
DOI: 10.3329/bjm.v27i1.9165