Plant Tissue Extraction Method for Complexed and Free Cyanide
نویسندگان
چکیده
منابع مشابه
Plant tissue extraction for metabolomics.
Plants are not only important producers of foods and energy storages (e.g., sugars, carbohydrates, proteins, and fats) in the form of grains, fruits, and vegetables, they also provide many valuable products to human existence including wood, fibers, oils, resins, pigments, antioxidants, and sources of medicine. Most importantly in light of this book, plants have been a source of therapeutic and...
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In the first part of this research, extensive speciesdependent cyanide recoveries studies were performed using the approved standard methods available for determination of free cyanide. The data obtained show that serious problems are associated with both the CATC (cyanide amenable to chlorination) and WAD (weak and dissociable cyanide) methods. In the second part, a novel flow injection gas-di...
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Soils on sites of former manufactured gas plants are usually contaminated with iron-cyanide complexes. We investigated the extraction of cyanide from 23 contaminated soils by three methods, which are prescribed by the German Federal Soil Protection Act and Ordinance: the soil-saturation extract with water, SSE, with a very narrow soil:solution ratio; the so-called S4 water extract with a soil:s...
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Fern gametophytes and young sporophytes often provide too little material for DNA extraction and are particularly difficult to identify to genus. Here we developed an efficient procedure called 'Tissue-direct PCR', in which a slice of fern tissue is mixed with PCR reagents and primers, allowing certain genomic regions to be amplified directly in the thermal cycler. For these diminutive and feat...
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In this study we present a simple and rapid method for tissue lipid extraction. Snap-frozen tissue (15-150 mg) is collected in 2 ml homogenization tubes. 500 μl BUME mixture (butanol:methanol [3:1]) is added and automated homogenization of up to 24 frozen samples at a time in less than 60 seconds is performed, followed by a 5-minute single-phase extraction. After the addition of 500 μl heptane:...
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ژورنال
عنوان ژورنال: Water, Air, & Soil Pollution
سال: 2004
ISSN: 0049-6979
DOI: 10.1023/b:wate.0000038905.46401.32