Plant Regeneration from Isolated Pollen Grains in Indica Type Rice(Oryza sativa L.).
نویسندگان
چکیده
منابع مشابه
Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.).
A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bas...
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Callus induction and regeneration in vitro of three rice varieties (Oryza sativa L. ssp indica), Bg 94-1, Moraberekon and Bg 300 were examined. Phenotypic variations of regenerated and parent plants were compared and the method of plant regeneration was confirmed by histology. Callusing was induced by culturing the mature embryos of the three rice varieties in a liquid medium of distilled water...
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Differential display gels were run for the drought tolerant (N-22) and drought susceptible (Panidhan) genotypes of rice (Oryza sativa) to identify the genes showing differential expression with respect to moisture stress. Differential cDNA products were cloned in PCR-Trap vector and analyzed for differential expression by Northern hybridization. Two clones namely R4A and R7G were found to be as...
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Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...
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Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells wh...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1992
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.42.675