منابع مشابه
Structure analysis of group I plant nucleases
Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several d...
متن کاملGenome editing in plant cells by zinc finger nucleases.
Gene targeting is a powerful tool for functional gene studies. However, only a handful of reports have been published describing the successful targeting of genome sequences in model and crop plants. Gene targeting can be stimulated by induction of double-strand breaks at specific genomic sites. The expression of zinc finger nucleases (ZFNs) can induce genomic double-strand breaks. Indeed, ZFNs...
متن کاملTranscription activator-like effector nucleases enable efficient plant genome engineering.
The ability to precisely engineer plant genomes offers much potential for advancing basic and applied plant biology. Here, we describe methods for the targeted modification of plant genomes using transcription activator-like effector nucleases (TALENs). Methods were optimized using tobacco (Nicotiana tabacum) protoplasts and TALENs targeting the acetolactate synthase (ALS) gene. Optimal TALEN s...
متن کاملStreptococcal Nucleases
Streptococcal DNAse C is more resistant to heat inactivation than the A or B enzyme. DNAses A and C are indifferent to the bacterial ribonucleic acid inhibitor whereas the B enzyme is markedly inhibited. Prolonged digestion with relatively large amounts of DNAse B results in chemical and biological destruction of the inhibitor. Ribonuclease as well as deoxyribonuclease activity is associated wi...
متن کاملSite-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding.
Conventional plant breeding exploits existing genetic variability and introduces new variability by mutagenesis. This has proven highly successful in securing food supplies for an ever-growing human population. The use of genetically modified plants is a complementary approach but all plant breeding techniques have limitations. Here, we discuss how the recent evolution of targeted mutagenesis a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1978
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.61.5.861