منابع مشابه
Dye loading with patch pipettes.
This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...
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When separating out those metals which are normally deposited on the cathode, a direct analysis of the amount deposited can be gotten by weighing the cathode before and after completion of electro-ultrafiltration, provided the current applied is above the deposition potential of the metal being removed. The collodion solutions can be prepared so as to give varying porosity according to the meth...
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Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manu...
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ژورنال
عنوان ژورنال: Journal of The Royal Society Interface
سال: 2018
ISSN: 1742-5689,1742-5662
DOI: 10.1098/rsif.2017.0868