Pistacia vera L. leaves as a renewable source of bioactive compounds via microwave assisted extraction

The production of pistachio in Tunisia generates a large amount potentially valuable waste, such as leaves, that could be used source bioactive compounds. In this work, the extraction phytochemicals from Pistacia vera L. leaves (male and female) by microwave-assisted (MAE) has been investigated. A response surface methodology (RSM) was for optimization compounds extraction, where independent variables were temperature time, dependent variable yield. Total phenolic content (TPC), total flavonoid (TFC), antioxidant activities (DPPH, ABTS, FRAP) antimicrobial activity extracts obtained at optimal conditions both evaluated compared not only between them, but also with maceration. optimum 70 °C 61 °C, 5.6 12 min male female respectivily. best results achieved MAE 36% yield, TPC 196.35 mg gallic acid equivalents (GAE)/g dry weight (DW), TFC 83.34 catechin (CE)/g high activities. evaluation biological showed provides better capacities then those Moreover, UPLC-DAD-ESI-MS performed to confirm presence some extracts, quercetin, apigenin myricetin derivatives. revealed is an efficient technique active components leaves.