Pinhole shifting lifetime imaging microscopy

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy

In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluore...

متن کامل

Imaging intracellular signaling using two-photon fluorescent lifetime imaging microscopy.

The recent development of Förster resonance energy transfer (FRET) sensors and FRET imaging techniques permits visualization of the dynamics of intracellular signaling events with high spatiotemporal resolution. In particular, fluorescence lifetime imaging in combination with two-photon laser-scanning microscopy (two-photon fluorescence lifetime imaging microscopy [2pFLIM]) is a powerful tool t...

متن کامل

Performing Enhanced Multiparameter Cell Imaging with Combined Fluorescence Lifetime Imaging Microscopy and Atomic Force Microscopy

optically encoded information about processes in live cells. Atomic force microscopy, on the other hand, provides nanometer-resolved surface topography and mechanical information, and has recently been expanded to nanometerresolved live cell mechanical property mapping. The integration of the two advanced live cell imaging techniques into one tool, with the capability to acquire simultaneous na...

متن کامل

Imaging beyond the Pinhole Camera

The world’s first photograph was taken by Joseph Nicphore Niépce (1775–1833) in 1826 on his country estate near Chalon-sur-Sane, France. The photo shows parts of farm buildings and some sky. Exposure time was eight hours. Niépce used a pinhole camera, known as camera obscura, and utilized pewter plates as the support medium for the photographic process. The camera obscura, the basic projection ...

متن کامل

Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP

Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution. Often ncomp kinetic processes underlie the observed fluorescence at all locations, but the intensity of the fluorescence associated with each process varies per-location, i.e., per-pixel imaged. Then t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Biomedical Optics

سال: 2008

ISSN: 1083-3668

DOI: 10.1117/1.3027503