Pigment content and molar extinction coefficients of photochemical reaction centers from Rhodopseudomonas spheroides
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چکیده
منابع مشابه
Protochlorophyll-like Pigment Produced by Rhodopseudomonas spheroides
The identification of intermediates in the biosynthesis of chlorophyll has been largely dependent upon the use ofmutants of Chlorella that are unable to synthesize chlorophyll but accumulate in the medium various tetrapyrrole compounds assumed to be precursors (for a review see Granick & Mauzerall, 1961). In particular, Granick (1950) identified magnesium vinylphaeoporphyrin a5 monomethyl ester...
متن کاملA partial reaction of delta-aminolaevulinate synthetase from Rhodopseudomonas spheroides.
microsomal fraction. The properties of the microsomal phosphorylcholinetransferase enzyme are similar to those reported for that from spinach leaves (Devor & Mudd, 1971), except that exogenous diglyceride was necessary for maximal rates, presumably because the activity of endogenous lipases was not high enough to generate a sufficient amount. These results indicate that, whereas the CDP-base pa...
متن کاملSpectral properties of reaction center preparations from Rhodopseudomonas spheroides.
The absorption, circular dichroism, and fluorescence spectra have been measured on oxidized and reduced reaction center preparations from Rhodopseudomonas spheroides strain R-26 at 295 and 77” K. The spectra indicate that the 4 bacteriochlorophyll a and 2 bacteriopheophytin a molecules in the reaction center are contained in a single pigment complex. The absorption bands at 893, 804, and ‘760 i...
متن کاملFluorescence studies on photosynthetic pigment development in Rhodopseudomonas spheroides.
When bleached, aerobically grown cells of Rhodopseudornonas spheroides are transferred to semi-aerobic conditions to induce bacteriochlorophyll synthesis, a new fluorescence band, with a maximum at 790 nm, is observed in addition to the 885 nm emission maximum normally seen in pigmented cells. The 790 nm fluorescence may be due to bacteriochlorophyll which has not been bound into the chromatoph...
متن کاملPrimary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of Rhodopseudomonas spheroides.
Reaction centers were found to bind two ubiquinones, both of which could be removed by o-phenanthroline and the detergent lauryldimethylamine oxide. One ubiquinone was more easily removed than the other. The low-temperature light-induced optical and electron paramagnetic resonance (EPR) changes were eliminated and restored upon removal and readdition of ubiquinone and were quantitatively corre...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 1973
ISSN: 0005-2728
DOI: 10.1016/0005-2728(73)90079-0