Phytochemical Screening and Chromatographic Purification of <i>Bauhunia semibifida</i> ROXB Hexane-Dichloromethane Leaf Extract

Bauhunia semibifida is among the several plants that have been studied for their medicinal properties. native to India. However, some species found in Africa. It has important uses, such as anti-bacterial, anti-inflammatory and anti-cancerous. This study aimed primarily at carrying out a preliminary phytochemical screening ascertain classes of bioactive compounds present solvent system(s) separation. Several solvents were used extraction well chromatographic profiling all products extraction. Thin layer chromatography was performed on silica gel-coated glass plates using mobile phase protocols (Hexane: Ethylacetate, Dichloromethane (DCM): Methanol). Further purification extracts done classical column (CcSiO2) gel filtration chromatography. TLC DCM Hexane shows same Rf values less than 1 are thus combined give Hexane/DCM extract, which gave two following isocratic elution from Phytochemical extract showed presence Tannins, Cardiac glycosides, Alkaloids, Flavonoids Phenolic compounds. contains phytocompounds can further be studied.