Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Abstract Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate phytochemical characterization two leaf extracts P. barbatus, their antimicrobial, antineoplastic immunomodulatory potential. After collection, herborization obtainment aqueous extract (PBA) acetone:water 7:3 organic (PBO), was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity determine minimum inhibitory concentration (MIC) against eight bacterial strains using microdilution test fungus Trichophyton rubrum disc diffusion assay test. Cytotoxicity assessed MTT trypan blue methods normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 100 µg.mL-1 neoplastic cell lines Toledo, K562, DU-145 PANC-1 1, 10 . Immunomodulatory activity, evaluated sandwich ELISA proinflammatory cytokines BALB/c mice splenocytes cultures supernatant. Both presented flavonoids, cinnamic derivatives, steroids ellagic acid. PBO showed bacteriostatic Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate PBA fungistatic 800 µg.mL-1). did not exhibit toxicity PBMCs (IC50 > Additionally, significantly inhibited IFN-γ IL-17A (p 0.03). potential candidate for use due its low healthy human exhibits biological activities medical interest as bacteriostatic, immunomodulatory.