Phytochemical Analysis, Cytotoxicity, Antibacterial and Antioxidant Activities of Extracts of Leaf of Ageratina adenophora (Spreng.)

The powdered leaf of Ageratina adenophora was subjected to extraction using a cold percolation method with methanol. After suspending the crude methanol extract in 1% HCl and neutralizing NH4OH, obtained solution extracted hexane, chloroform, ethyl acetate solvent, respectively. Qualitative phytochemical analysis methanol, extracts A. plant showed presence alkaloids, flavonoids, phenols, steroids, quinones, saponins, tannins, cardiac glycosides, carbohydrate, terpenoids, proteins, amino acids. GC-MS chloroform 10 different major compounds which α-Muurolol (24.33%) found most abundant. IC50 value be 1460 µg/mL from DPPH scavenging antioxidant assay, LC50 174.78 brine shrimp lethality assay. Antibacterial activity shown highest against Escherichia coli Proteus vulgaris ZOI 12 mm on each extract. It determined that 89.75 mg gallic acid equivalent/g dry accounted up total phenolic content 49.25 quercetin observed flavonoid