Physiochemical Properties of Rat Liver Mitochondrial Ribosomes
نویسندگان
چکیده
منابع مشابه
A Structural Study of Rat Liver Ribosomes
Polyribosomes, ribosomes, and ribosomal subunits were prepared from rat liver using sodium deoxycholate and a variety of ionic media. They were examined in the electron microscope, mainly as negatively or positively stained preparations, and in the analytical ultracentrifuge. The polyribosomes consist of up to twelve or more ribosomes linked by a fine strand, 10 to 15 A in diameter, probably of...
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Amino acid incorporation by isolated intact rat liver mitochondria was severely inhibited by chloramphenicol, carbomycin, or sparsomycin, partially inhibited by emetine, and unatIected by erytbromycin or lincomycin. By contrast, amino acid incorporation by inner membrane-matrix fractions prepared from rat liver mitochondria by digitonin was strongly inhibited by emetine, erythromycin, and linco...
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1. Polynucleotide phosphorylase was partially purified from the inner membrane of rat liver mitochondria. 2. The partially purified particulate enzyme catalyses phosphorolysis of poly(A), poly(C), poly(U) and RNA to nucleoside diphosphates. 3. It is devoid of nucleoside diphosphate-polymerization activity. 4. Variable amounts of ADP/P(i)-exchange activity are associated with the polynucleotide ...
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Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cytosol proteins. In contrast, translation products of poly A+RNA from membrane-bound polyribosomes ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m005781200