Physicochemical characterization of agricultural run-off and groundwater inoculated by Trichoderma asperellum and its effect on anti-oxidative enzymes production by irrigated Trifolium alexandrinum L.

Water safety and security has become a great problem in recent years, as the shortage of water resources most countries is becoming an increasingly serious issue. Therefore, searching for new sources or treating contaminated major requirement to covering fresh water. The current study evaluated impact Trichoderma asperellum on physicochemical characterization agricultural run-off (AR) compared with groundwater (GW). Chemical oxygen demand (COD), biological (BOD5), ammonia, total nitrogen (TN), dissolved salts (TDS) AR were higher than that GW 90, 90.2, 91.9, 78, 61.6%, respectively. addition T. caused COD, BOD5, TN, TDS decrease 47.2, 39.2, 16.2, 12.9, 4.8%, Contents metals decreased by asperellum. Microbial analysis soil irrigated contrast showed presence high number bacteria (32 × 106 CFU), actinomycetes (18 104 fungi (13 CFU). Trifolium alexandrinum growth its antioxidant enzymes activities (catalase, superoxide dismutase, ascorbate peroxidase) enhanced inoculated un-inoculated GW.