Physical Maps of Autographa californica and Rachiplusia ou Nuclear Polyhedrosis Virus Recombinants
نویسندگان
چکیده
منابع مشابه
Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses.
The Rachiplusia ou multiple nucleopolyhedrovirus (RoMNPV) is a variant of Autographa californica MNPV (AcMNPV) but is significantly more virulent against several major agricultural pests. The genome sequence of the R1 strain of RoMNPV was determined and compared to that of AcMNPV strain C6. The RoMNPV genome is approximately 131.5 kbp with a G+C content of 39.1 %. The homologous repeat regions ...
متن کاملAutographa californica nuclear polyhedrosis virus DNA polymerase: measurements of processivity and strand displacement.
The DNA polymerase (DNApol) of Autographa californica nuclear polyhedrosis virus was purified to homogeneity from recombinant baculovirus-infected cells. DNApol was active in polymerase assays on singly primed M13 template, and full-length replicative form II product was synthesized at equimolar ratios of enzyme to template. The purified recombinant DNApol was shown to be processive by template...
متن کاملEffects of Temperature and pH on Survival of Free Nuclear Polyhedrosis Virus of Autographa californica.
The effects of temperature and low pH on replication and survival of nonoccluded Autographa californica nuclear polyhedrosis virus were investigated. No virus replication or formation of polynuclear inclusion bodies occurred at 37 degrees C. The virus was immediately inactivated upon exposure to pH 2.0 and was inactivated within 1 h at pH 4.0. The virus titer slowly declined, a 3-orders of magn...
متن کاملTime Course Analysis and Mapping of Autographa californica Nuclear Polyhedrosis Virus Transcripts.
To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continued to be synthesized until late in infection. Polyhedrin and p8 mRNAs were the...
متن کاملIdentification of seven putative origins of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA replication.
Seven putative origins of DNA replication (oris) were identified and located on the genome of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV), when an improved infection-dependent replication assay was used. A threefold higher yield of amplified plasmid was achieved when an m.o.i. of 1 was used (instead of 25), and another twofold increase was obtained when the ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1980
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.34.3.693-703.1980